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NHA Phlebotomy-Technician Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Special Collections12%- Glucose tolerance & dynamic tests
- Pediatric & geriatric collections
- Blood cultures & sterile collection
- Blood alcohol & legal collections
- Therapeutic drug monitoring
Topic 2: Patient Preparation20%- Informed consent & communication
- Requisition review & test verification
- Site selection & preparation
- Patient positioning & comfort
- Patient identification & verification
Topic 3: Specimen Processing & Handling14%- Documentation & tracking
- Specimen rejection criteria
- Processing & centrifugation
- Transport & storage requirements
- Aliquoting & preservation
Topic 4: Routine Blood Collections28%- Post-collection care & labeling
- Tourniquet use & vein assessment
- Venipuncture techniques & equipment
- Order of draw & tube selection
- Capillary puncture procedures
Topic 5: Safety and Compliance26%- Bloodborne pathogens & exposure control
- Infection control & OSHA standards
- HIPAA & patient confidentiality
- Quality control & error prevention
- PPE & sharps disposal

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NHA Phlebotomy Technician Certification Exam (CPT) Sample Questions (Q104-Q109):

NEW QUESTION # 104
Which of the following sites should a phlebotomist use for collecting a capillary blood specimen from an adult patient?

Answer: B

Explanation:
For an adult capillary blood specimen, the third digit with the cut perpendicular to the fingerprint grooves is the best answer. The third digit is the middle finger, one of the preferred fingers for dermal puncture because it usually has adequate tissue depth and blood flow. The incision should be made perpendicular to the fingerprint lines so blood forms a rounded drop instead of running along the fingerprint grooves, which improves collection efficiency and reduces specimen smearing. The second digit, or index finger, is generally avoided because it may be more calloused or sensitive and is used frequently for touch. The fifth digit should be avoided because it has less tissue depth and greater risk of bone injury. A cut parallel to fingerprint grooves is technically poor because blood can track along the grooves and make collection more difficult. Correct adult capillary collection requires site warming when needed, antisepsis, complete drying, appropriate lancet depth, wiping away the first drop when required, and avoiding excessive squeezing. Reference topics: Routine Blood Collections; dermal puncture; adult capillary site selection; lancet orientation; microcollection technique.


NEW QUESTION # 105
Which of the following tube additives promotes clot formation?

Answer: A

Explanation:
Silica promotes clot formation and is commonly used as a clot activator in serum separator tubes and other serum collection tubes. Its function is to accelerate the clotting process so serum can be separated after appropriate clotting time and centrifugation. Heparin is the opposite; it is an anticoagulant that inhibits thrombin activity and is used when plasma is required, especially for many chemistry tests. Oxalate is also an anticoagulant, commonly paired with sodium fluoride in gray-top tubes, where oxalate inhibits clotting and fluoride helps preserve glucose by inhibiting glycolysis. Citrate is another anticoagulant, usually in light-blue tubes for coagulation testing, where the blood-to-additive ratio is critical. This question tests additive function, not tube color memorization alone. A phlebotomist must know whether a tube is intended to produce serum, plasma, or whole blood because incorrect tube selection directly creates preanalytical error and possible specimen rejection. Reference topics: Routine Blood Collections; tube additives; clot activators; order of draw; specimen integrity. NHA's CPT test plan includes equipment, collection tubes, additives, and routine blood collection procedures.


NEW QUESTION # 106
A patient's recent consumption of fatty substances could result in which of the following elevated analytes?

Answer: B

Explanation:
Recent consumption of fatty substances can elevate lipid-related analytes and can also produce lipemia, a milky or turbid appearance in serum or plasma. Therefore, the correct answer is lipid. This is why fasting status is important for certain chemistry tests, especially lipid panels or triglyceride-related testing. If the patient has not followed fasting requirements, the specimen may not reflect basal-state values and can produce misleading results. Bilirubin is primarily associated with hemoglobin breakdown and liver or biliary function; it is not directly elevated simply because the patient recently ate fatty food. Albumin is a plasma protein reflecting liver synthesis, nutrition, inflammation, or fluid status, not immediate dietary fat intake. Lipase is a pancreatic enzyme used mainly in the evaluation of pancreatic disorders and is not the same as lipids. The phlebotomist's responsibility is to verify patient compliance with fasting, medication, timing, and basal-state requirements before collection and proceed according to facility policy. Reference topics: Patient Preparation; fasting requirements; medication and basal-state compliance; preanalytical variables.


NEW QUESTION # 107
Which of the following questions should a phlebotomist ask to verify fasting status?

Answer: B

Explanation:
The phlebotomist should ask, "When was the last time you had anything to eat or drink other than water?" This question directly verifies fasting status and avoids a vague yes/no answer. Patients may misunderstand fasting and think coffee, juice, gum, candy, or a small snack does not count. Asking about medication is important for some tests, but it does not verify fasting. Asking why the provider ordered the test is outside the phlebotomist's interpretive role and may lead to unauthorized clinical discussion. Asking whether the patient has had the test before does not confirm current preparation. Accurate fasting verification is part of patient preparation and preanalytical quality control. If the patient has not fasted as required, the phlebotomist should follow facility policy, notify appropriate personnel, document noncompliance if collection proceeds, or reschedule if instructed. Poor preparation can produce misleading chemistry results and unnecessary repeat testing. Reference topics: Patient Preparation; fasting verification; patient interview; basal-state requirements; preanalytical variables.


NEW QUESTION # 108
Which of the following additives is commonly found in a gray-top tube used for glucose testing?

Answer: C

Explanation:
Sodium fluoride is commonly found in gray-top tubes used for glucose testing. Sodium fluoride inhibits glycolysis, which helps preserve glucose concentration after collection by slowing cellular glucose consumption. Many gray-top tubes also contain potassium oxalate as an anticoagulant. Sodium citrate is found in light-blue tubes used for coagulation studies because it binds calcium reversibly. EDTA is found in lavender-top tubes and is commonly used for hematology testing such as CBC. SPS is sodium polyanethol sulfonate, an additive used in blood culture bottles or yellow SPS tubes because it supports organism recovery by reducing certain inhibitory effects. Tube additives are not trivia; they determine specimen type and test validity. A glucose specimen collected in the wrong tube or processed late may produce inaccurate results because cells continue metabolizing glucose after collection. The phlebotomist must match test, tube, additive, order of draw, fill volume, and handling requirements. Reference topics: Special Collections; glucose testing; gray-top tube; sodium fluoride; additive function.


NEW QUESTION # 109
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