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| Section | Objectives |
|---|---|
| Topic 1: Patient Preparation | - Specimen labeling procedures - Patient identification and communication - Site selection and preparation |
| Topic 2: Safety and Compliance | - OSHA regulations and patient safety - HIPAA and patient confidentiality - Infection control and standard precautions |
| Topic 3: Specimen Processing and Handling | - Specimen transport and storage - Common errors and rejection criteria - Quality assurance procedures |
| Topic 4: Special Collections | - Non-routine specimen collection methods - Pediatric and geriatric considerations - Capillary puncture (fingerstick/heel stick) |
| Topic 5: Routine Blood Collections | - Order of draw - Venipuncture procedures - Equipment selection and use |
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NEW QUESTION # 119
Which of the following is the maximum allowed time for a tourniquet application before possibly affecting test results?
Answer: D
Explanation:
The maximum recommended tourniquet application time before results may be affected is 60 seconds. A tourniquet is applied to increase venous filling and make the vein easier to locate and access, but prolonged application causes venous stasis and hemoconcentration. When blood remains trapped below the tourniquet too long, fluid shifts from the vascular space into surrounding tissue, concentrating cells, proteins, and protein-bound analytes. This can falsely elevate results such as potassium, calcium, proteins, and other chemistry values. It can also increase patient discomfort and make the collection more difficult. Thirty and forty-five seconds are acceptable but are not the maximum allowed time. Ninety seconds is too long and increases the risk of altered test values. Correct practice is to apply the tourniquet only long enough to select the site and establish blood flow, then release it promptly, especially before removing the needle. Reference topics: Routine Blood Collections; tourniquet application; hemoconcentration; venipuncture preanalytical variables.
NEW QUESTION # 120
A phlebotomist is preparing to collect a CBC and a basic metabolic panel using evacuated tubes. Which of the following tubes should the phlebotomist collect first?
Answer: A
Explanation:
For a CBC and basic metabolic panel, the green-top tube should be collected before the lavender-top tube when following the standard order of draw after sterile/blood culture tubes and coagulation tubes. A basic metabolic panel is commonly collected in a green-top heparin tube or serum tube depending on facility protocol, while a CBC requires a lavender-top EDTA tube. Additive carryover is the key concept. EDTA from a lavender tube can contaminate later specimens and falsely elevate potassium while lowering calcium because EDTA binds calcium. This makes lavender-top collection before chemistry specimens technically unsafe when chemistry testing is also ordered. Gray-top tubes are generally used for glucose or lactate testing and are collected later. Yellow SPS tubes are used for blood cultures and would be collected first only if blood cultures were ordered. The phlebotomist must know order of draw, tube additives, and the clinical consequence of additive contamination. Reference topics: Routine Blood Collections; order of draw; tube additives; CBC; chemistry specimen collection.
NEW QUESTION # 121
A blood specimen is rejected by the laboratory for testing. Which of the following actions should the phlebotomist take?
Answer: B
Explanation:
The phlebotomist should contact the patient to repeat the venipuncture collection, following facility policy and provider notification procedures. A rejected specimen cannot be tested reliably, so the practical correction is recollection using the proper tube, volume, labeling, handling, and transport requirements. Waiting until the next yearly visit is unacceptable because the ordered test was needed for current clinical care. Shredding documentation is improper unless performed under approved records-management procedures, and it does not solve the missing valid specimen. Sending laboratory results by mail is impossible or inappropriate when the specimen was rejected and no valid result exists. Specimen rejection usually results from preanalytical problems such as wrong tube, insufficient volume, hemolysis, clotting in an anticoagulant tube, unlabeled or mislabeled specimen, expired supplies, improper temperature, or delayed transport. The phlebotomist's job is to correct the collection failure promptly and prevent recurrence. Reference topics: Processing Specimens; specimen rejection; recollection; preanalytical error; specimen integrity.
NEW QUESTION # 122
Which of the following additives is commonly found in a gray-top tube used for glucose testing?
Answer: B
Explanation:
Sodium fluoride is commonly found in gray-top tubes used for glucose testing. Sodium fluoride inhibits glycolysis, which helps preserve glucose concentration after collection by slowing cellular glucose consumption. Many gray-top tubes also contain potassium oxalate as an anticoagulant. Sodium citrate is found in light-blue tubes used for coagulation studies because it binds calcium reversibly. EDTA is found in lavender-top tubes and is commonly used for hematology testing such as CBC. SPS is sodium polyanethol sulfonate, an additive used in blood culture bottles or yellow SPS tubes because it supports organism recovery by reducing certain inhibitory effects. Tube additives are not trivia; they determine specimen type and test validity. A glucose specimen collected in the wrong tube or processed late may produce inaccurate results because cells continue metabolizing glucose after collection. The phlebotomist must match test, tube, additive, order of draw, fill volume, and handling requirements. Reference topics: Special Collections; glucose testing; gray-top tube; sodium fluoride; additive function.
NEW QUESTION # 123
Which of the following tube colors is commonly used for coagulation testing?
Answer: D
Explanation:
Light-blue tubes are commonly used for coagulation testing because they contain sodium citrate. Citrate binds calcium reversibly, preventing clotting while preserving the specimen for coagulation analysis such as PT, INR, and aPTT. The tube must be filled to the required level because coagulation testing depends on a precise blood-to-anticoagulant ratio. Underfilling a light-blue tube can falsely prolong clotting results and cause specimen rejection. Lavender tubes contain EDTA and are commonly used for hematology tests such as CBC. Gray tubes often contain sodium fluoride and potassium oxalate and are used for glucose or lactate testing depending on facility protocol. Red tubes are generally serum tubes without anticoagulant or with clot activator depending on manufacturer. The phlebotomist must collect light-blue tubes in the correct order of draw and inspect for proper fill. This is a high-yield NHA-style concept because it combines tube color, additive, test department, and specimen acceptability. Reference topics: Routine Blood Collections; coagulation tubes; sodium citrate; fill volume; order of draw.
NEW QUESTION # 124
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