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NHA Phlebotomy-Technician Exam Syllabus Topics:

SectionObjectives
Topic 1: Safety and Compliance- HIPAA and patient confidentiality
- OSHA regulations and patient safety
- Infection control and standard precautions
Topic 2: Routine Blood Collections- Venipuncture procedures
- Equipment selection and use
- Order of draw
Topic 3: Special Collections- Pediatric and geriatric considerations
- Non-routine specimen collection methods
- Capillary puncture (fingerstick/heel stick)
Topic 4: Patient Preparation- Site selection and preparation
- Specimen labeling procedures
- Patient identification and communication
Topic 5: Specimen Processing and Handling- Common errors and rejection criteria
- Quality assurance procedures
- Specimen transport and storage

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NHA Phlebotomy Technician Certification Exam (CPT) Sample Questions (Q100-Q105):

NEW QUESTION # 100
Which of the following tests is most likely to require collection in a chilled specimen tube?

Answer: B

Explanation:
Ammonia is most likely to require collection in a chilled specimen tube and rapid transport, depending on facility protocol. Ammonia is unstable and can be affected by delayed processing, temperature, and cellular metabolism after collection. Chilling slows metabolic activity and helps preserve specimen integrity. Total cholesterol is generally a routine chemistry test and does not usually require immediate chilling. Hemoglobin A1c is collected to evaluate long-term glycemic control and is not typically a chilled specimen. Blood cultures require aseptic technique, correct culture bottles, adequate volume, and timely incubation or transport, not chilling. The broader lesson is that special handling requirements are analyte-specific. Some specimens must be chilled, some kept warm, some protected from light, and some delivered immediately. The phlebotomist must review the requisition, laboratory directory, or facility procedure before collection. Improper temperature handling can produce inaccurate results even if the specimen was collected in the correct tube. Reference topics: Special Collections; chilled specimens; ammonia handling; specimen stability; transport requirements.


NEW QUESTION # 101
A phlebotomist notices that a serum separator tube has not clotted before centrifugation. Which of the following actions should the phlebotomist take?

Answer: C

Explanation:
The phlebotomist should allow the serum separator tube to clot completely before centrifugation. Serum specimens require clot formation before centrifugation so the liquid serum can separate properly from the clot. Centrifuging before clotting is complete can cause fibrin strands, incomplete separation, instrument issues, and possible specimen rejection. Shaking the tube vigorously is incorrect because it can hemolyze the specimen and disrupt cellular integrity. Transferring the specimen into a plasma tube is unsafe and invalid because the specimen was not collected with the correct anticoagulant at the correct blood-to-additive ratio. Proper processing means respecting the requirements of the tube collected: serum tubes must clot for the required time, additive tubes must be gently inverted, and specimens must be centrifuged according to time, speed, and temperature requirements. This item tests processing discipline, not speed. A phlebotomist must protect specimen integrity from collection through transport and processing. Reference topics: Processing Specimens; serum separation; clotting time; centrifugation; specimen rejection.


NEW QUESTION # 102
Which of the following collections requires the phlebotomist to discard the first voided specimen and save all subsequent urine for a full day?

Answer: B

Explanation:
A 24-hour urine collection requires the patient to discard the first voided specimen and then save all subsequent urine for the full collection period, including the final void at the ending time. Discarding the first void empties the bladder and establishes the start of the timed interval. A clean-catch urine culture requires cleansing and collecting a midstream specimen in a sterile container, not saving urine for 24 hours. A random urinalysis is collected at a single time and does not require timed accumulation. A sputum culture is a respiratory specimen, not a urine collection. Patient instruction is critical because one missed urine specimen can invalidate the entire 24-hour collection. The phlebotomist should explain start and stop times, storage requirements, preservative hazards if present, labeling, and return instructions. The patient should not change fluid intake unless instructed by the provider. Reference topics: Special Collections; 24-hour urine; patient instructions; non-blood specimens; timed collection.


NEW QUESTION # 103
A phlebotomist has just performed quality control measurements on a glucose monitor. The values exceed the acceptable limits. Which of the following actions should the phlebotomist take?

Answer: D

Explanation:
When glucose monitor quality control values exceed acceptable limits, the phlebotomist should perform the manufacturer's recommended actions. Out-of-range QC means the instrument, strips, controls, operator technique, storage conditions, or environmental factors may be unreliable. Patient testing should not proceed until the problem is corrected and QC results return to acceptable range according to the device instructions and facility procedure. Cleaning the monitor may be part of troubleshooting, but it is not automatically the complete or correct response unless the manufacturer's protocol identifies contamination as the cause. Replacing batteries may be appropriate only if the device indicates low power or the manufacturer's instructions direct it. Documenting the values is necessary, but documentation alone does not correct the failed QC or validate patient results. The correct professional action is to follow the device-specific troubleshooting and QC procedure, repeat controls when indicated, remove defective materials from use, and notify the appropriate supervisor if unresolved. NHA CPT competencies include point-of-care testing, quality control, CLIA-waived testing, equipment maintenance, and accurate documentation. Reference topics: Special Collections; point-of-care glucose testing; quality control; manufacturer instructions.


NEW QUESTION # 104
A phlebotomist must recollect a blood sample after the initial sample was rejected by the laboratory. Which of the following factors might have caused the sample to be rejected?

Answer: D

Explanation:
An underfilled collection tube is a common reason for laboratory rejection because many tests require a specific blood-to-additive ratio. This is especially critical for coagulation specimens, where insufficient blood relative to anticoagulant can falsely prolong clotting results. Underfilling can also produce inadequate specimen volume for testing, repeat analysis, or instrument requirements. A hematoma at the puncture site is a patient complication and may indicate procedural difficulty, but it does not automatically require specimen rejection unless it affected specimen quality. Use of a multiple-sample needle is a standard venipuncture method and is not a rejection criterion by itself. The cephalic vein is an acceptable antecubital vein when selected appropriately, so using it does not invalidate the specimen. Processing accuracy begins at collection: correct tube, correct volume, correct mixing, correct labeling, and correct transport. The NHA CPT outline identifies preanalytical errors, minimum and maximum volume requirements, fill levels and ratios, maintaining specimen integrity, and recollection as core processing and collection competencies. Reference topics: Processing Specimens; preanalytical errors; tube fill level; specimen rejection and recollection.


NEW QUESTION # 105
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